In: Journal of Cell Science, 103 (1992), S. 811-822
Abstract
Invariant chain (Ii) associated with MHC class II molecule is processed proteolytically via several distinct intermediates during its intracellular transport through endosomal compartments. Leupeptin added to the culture medium blocks processing of Ii, prevents its dissociation from the class II molecules and leads to an intracellular accumulation of a 22 kDa intermediate form of Ii. We show here that leupeptin has a very general effect on protein transport in the endocytic pathway. When added to Mel Juso cells leupeptin reduces the transport of endocytosed material from multivesicular body-like, endosome carrier vesicles (ECV) to the prelysosomal compartment (late endosome) and leads to a concomitant increase in the number of ECV. Our results argue that one effect of leupeptin, related to antigen processing and presentation, is to block transport of antigen and/or MHC class II molecules to prelysosomal compartments.
Dokumententyp: | Artikel |
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Titel der Zeitschrift: | Journal of Cell Science |
Band: | 103 |
Erstellungsdatum: | 15 Jul. 2008 17:23 |
Erscheinungsjahr: | 1992 |
Seitenbereich: | S. 811-822 |
Institute/Einrichtungen: | Zentrale und Sonstige Einrichtungen > Zentrum für Molekulare Biologie (ZMBH) |
DDC-Sachgruppe: | 570 Biowissenschaften, Biologie |
Freie Schlagwörter: | leupeptin , invariant chain (Ii) , endosome carrier vesicle (ECV) , endocytosis |