In: The Journal of Cell Biology, 121 (1993), Nr. 5. S. 977-985
Vorschau |
PDF, Englisch
Download (1MB) | Nutzungsbedingungen |
Abstract
Signal recognition particle (SRP), the cytoplasmic ribonucleoprotein particle that mediates the targeting of proteins to the ER, consists of a 7S RNA and six different proteins. The 68- (SRP68) and 72-(SRP72) kD proteins of SRP are bound to the 7S RNA of SRP as a heterodimeric complex (SRP68/72). Here we describe the primary structure of SRP72 and the assembly of SRP68, SRP72 and 7S RNA into a ribonucleoprotein particle. The amino acid sequence deduced from the cDNA of SRP72 reveals a basic protein of 671 amino acids which shares no sequence similarity with any protein in the sequence data libraries. Assembly of SRP72 into a ribonucleoprotein particle required the presence of 7S RNA and SRP68. In contrast, SRP68 alone specifically bound to 7S RNA. SRP68 contacts the 7S RNA via its NH2-terminal half while COOH-terminal portions of SRP68 and SRP72 are in contact with each other in SRP.SRP68 thus serves as a link between 7S RNA and SRP72. As a large NH2-terminal domain of SRP72 is exposed on SRP it may be a site of contact to other molecules involved in the SRP cycle between the ribosome and the ER membrane.
Dokumententyp: | Artikel |
---|---|
Titel der Zeitschrift: | The Journal of Cell Biology |
Band: | 121 |
Nummer: | 5 |
Erstellungsdatum: | 17 Jul. 2008 15:23 |
Erscheinungsjahr: | 1993 |
Seitenbereich: | S. 977-985 |
Institute/Einrichtungen: | Zentrale und Sonstige Einrichtungen > Zentrum für Molekulare Biologie (ZMBH) |
DDC-Sachgruppe: | 570 Biowissenschaften, Biologie |
Schriftenreihe: | Schriften von Bernhard Dobberstein |