In: The EMBO Joumal, 1 (1982), Nr. 4. S. 467-471
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Abstract
A cDNA clone known to code for a mouse histocompatibility (class I) antigen was found to contain a sequence specific for a subpopulation of H-2 genes. This unique sequence is located in the 3' non-coding region close to the stretch of poly(A) nucleotides. A subclone containing this fragment(pH-2d-5) has been used to select hybridizing mRNA. Translation of the mRNA in vitro shows that H-2Kd mRNA is selected. Southern blot analysis of DNA from congenic recombinant mice show that at least one gene containing this sequence is located at the K locus (region) of the major histocompatibility complex. This gene contains a 3.7-kb Bgml and a 13-kb EcoRI restriction endonuclease fragment. This gene has been isolated from a genomic DNA library.
| Dokumententyp: | Artikel |
|---|---|
| Titel der Zeitschrift: | The EMBO Joumal |
| Band: | 1 |
| Nummer: | 4 |
| Erstellungsdatum: | 02 Mrz. 2009 11:01 |
| Erscheinungsjahr: | 1982 |
| Seitenbereich: | S. 467-471 |
| Institute/Einrichtungen: | Zentrale und Sonstige Einrichtungen > Zentrum für Molekulare Biologie (ZMBH) |
| DDC-Sachgruppe: | 570 Biowissenschaften, Biologie |
| Freie Schlagwörter: | histocompatibility genes , hybrid-selected translation , gene mapping , 3' non-coding region, Southern blot analysis |
| Schriftenreihe: | Schriften von Bernhard Dobberstein |
