German Title: Kombination von strukturierter Beleuchtung und Lokalisationsmikroskopie in einem neuen Setup mit Anwendungen an retinale Strukturen
Fluorescence microscopy methods have become an major imaging tool in biomedical lifescience. However, each method only addresses specific questions due to intrinsic limitations. A new, fully automated and user-friendly 'Combo'-microscope setup has been developed, which combines the two advanced high-resolution methods Structured Illumination and Localization Microscopy into one imaging system. As both methods complement one another, the 'Combo'-microscope will greatly extend the range of application in biomedical research. Moreover, artifacts, introduced in the course of the imaging and/or reconstruction processes, can be revealed and potential mis-interpretation of super-resolution data is limited. This work was motivated by the age-related macular degeneration (AMD), a disease, which is the leading cause of blindness in the Western world. Autofluorescent particles within age-related deposits (drusen) beneath the retinal pigment epithelium are studied on advanced resolution level. Furthermore, the newly built microscope is used for a dual-mode dual-color three-dimensional visualization of the axon initial segment, a crucial region for signal transduction in vision, of retinal ganglion cells. Finally, an in vitro study comparing the pharmaceuticals currently used for AMD-treatment is outlined.
|Supervisor:||Cremer, Prof. Dr. Christoph|
|Date of thesis defense:||18 June 2014|
|Date Deposited:||07 Jul 2014 08:10|
|Faculties / Institutes:||The Faculty of Physics and Astronomy > Kirchhoff Institute for Physics|