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Deep tissue light-sheet microscopy

Quintas Glasner de Medeiros, Gustavo

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Abstract

Light-sheet fluorescence microscopy, also recognised as selective plane illumination microscopy, or SPIM, has paved a new road towards imaging of entire specimens for long periods of time, in vivo. Nevertheless, as in any other microscopy technique, light-sheet fluorescence microscopy also heavily depends on the scattering and absorption properties of the imaged sample in order to generate 3D datasets with high signal to noise even at larger tissue depths. This thesis focuses on the development and implementation of new strategies and methods which target the minimization of scattering and absorption effects stemming from living specimens. Combined, the three methods provide the ability to perform gentle, high contrast deep tissue imaging and photomanipulation. Additionally, it allows easier handling and fusion of 3D multiview light-sheet images.

Item Type: Dissertation
Supervisor: Knop, Prof. Dr. Michael
Place of Publication: Heidelberg
Date of thesis defense: 16 September 2016
Date Deposited: 06 Oct 2017 09:25
Date: 2017
Faculties / Institutes: The Faculty of Bio Sciences > Dean's Office of the Faculty of Bio Sciences
Subjects: 500 Natural sciences and mathematics
530 Physics
570 Life sciences
600 Technology (Applied sciences)
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